13 pages. Covers collection requirements for eight sterile site specimen types, a seven step rule for allocating an insufficient and unrepeatable volume, direct examination as the highest yield step, and concentration and bottle inoculation for low organism burden. Includes culture and incubation protocols by specimen including fourteen day prosthetic joint work, periprosthetic tissue sampling, dialysis effluent, interpretation of growth against a sterile site standard, and handling of broth only growth.